Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2 . 0mg / l 6 - ba and l . omg / l naa . the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods 通过将不同浓度的秋水仙碱加入培养基和不同浓度的秋水仙碱溶液浸泡两种方法处理愈伤组织，从而筛选出最佳的诱导方法和最佳的秋水仙碱浓度和处理时间。
